ConFix
ConFix is a new generation product from Proscix, designed for multi dimensional and multi parameter studies of biological samples. Combining label free fluorescence, differential static light scattering and dynamic light scattering technologies, ConFix characterizes protein status in 48 well plates under defined temperature conditions.
ConFix supports a wide range of applications
- 1Protein thermal stability analysis in temperature ramp scanning or isothermal experiments
- 2Formulation development for therapeutic monoclonal antibodies
- 3Membrane protein characterization
- 4Comparative studies of ligand specificity, e.g. substrates, co factors and inhibitors
- 5Validation of high throughput screening (HTS) hits
- 6Quality control (QC) of protein biotherapeutics
- 7Focused library screening for buffer optimization, protein crystallization and chemical biology research
- 8Stability comparison of SNP proteins
- 9Investigation of protein structural and aggregation stability under isothermal conditions via full spectrum intrinsic fluorescence and static light scattering. It can simulate physiological conditions, accelerate and complement conventional incubator based assays, and support accelerated stability and long term stability studies of proteins
Label Free Technology
ConFix determines protein structural stability via fluorescence spectra and protein aggregation via static light scattering. It imposes no constraints on buffer compositions and performs measurements even in detergent environments. No fluorescent dyes are required; universal protein dyes such as ANS may be added optionally. For samples that are difficult to screen by conventional DSF, label free static light scattering on ConFix readily determines Tagg, from which Tm and Tonset can be calculated. Tm calculation algorithms include emission peak method, 350/330 dual wavelength ratio method, area under curve method and centroid wavelength method.
- Temperature control range: 20 ~ 95 °C
- Capillary based format; maximum heating rate up to 10 °C/min with short cooling time, delivering high throughput and fast results.
- Sample volume as low as 2.5 μL
- Compatible with standard low volume 48 well optical bottom microplates. ConFix collects data for up to 48 samples per run (24 wells are reserved for instrument use). To conserve precious reagents, aggregation can be measured with sample volumes down to 2.5 μL per well. Typical protein concentration range: 0.05 mg/mL ~ 200 mg/mL.
Advanced Software
Method driven experiments within the control software allow users to customize instrument operation according to requirements. Automated data processing enables rapid result analysis. Data export is compatible with Excel, Bioactive and LIMS. Optional online software upgrades deliver the latest user experience enhancements.
REAL OS System
Real time operating status of ConFix is clearly indicated by a predefined colour coded system on the REAL OS user interface. Its intuitive layout facilitates effortless navigation. Thanks to web enabled connectivity and web browser architecture, the instrument can be accessed from any computer on the corporate network for continuous status monitoring. The integrated REAL OS software automatically manages all operating modes via customizable run schedules for user defined operation timelines, enabling seamless integration into daily laboratory workflows. Automatic validation of all relevant parameters means users only need to define workflow steps; the instrument completes the rest autonomously. Comprehensive user group management and audit trace functionality render ConFix compliant with FDA 21 CFR Part 11 and ISO 17025 standards, making it ideal for regulated laboratories.
Easy Sample Handling
ConFix delivers reliable, precise analytical results for up to 48 samples per run across diverse sample matrices, whether solid or liquid.
High Operational Safety
Robust construction combined with tool free routine handling delivers intuitive, safe operation for all users.
Accurate Cost Accounting
ConFix accepts helium or argon as carrier gas. Durable core components and high quality consumables enable well planned overall operating cost management.
Intuitive Operating Experience
The user interface guides users through complete analytical workflows. Customizable procedures and parameter settings simplify daily laboratory routines.
Flexible Equipment Availability
ConFix operates independently of working hours to boost laboratory productivity. Automated self diagnostics guarantee safe unattended operation.
Customized Maintenance Solutions
All instrument components are engineered for high reliability. Tailored maintenance and service packages are nevertheless essential. Our service team provides expert consultation upon request.
Application Technical Support
Leverage our professional expertise and collaborate with our laboratory team to develop custom analytical workflows for your applications.
Broad Sample Compatibility Enables Unprecedented Experimental Flexibility
Whether working with purified proteins, cell lysates, antibody therapeutics, high concentration formulations or dilute samples, the ConFix protein stability analyser delivers robust, accurate melting temperature (Tm) and aggregation behaviour data for up to 48 samples in a single run. Its patented autosampler prevents sample mix ups and ensures full traceability. Flexible run queue management allows emergency samples to be inserted without interrupting ongoing measurements.
One day you analyse high concentration mAb samples at 80 mg/mL; the next day you switch to low concentration enzyme solutions at 70 mg/mL or viscous formulations — ConFix copes effortlessly. Optimized temperature control and optical detection systems permit direct loading of samples with varying buffer compositions, viscosities and turbidity without extra pre treatment. Benefits include true "load and measure" high throughput stability screening, improved R&D efficiency and long term stable instrument performance.
Maintenance Related Downtime? Not for ConFix!
Through thoughtful engineering, ConFix sets industry benchmarks for equipment availability, serviceability and durability. All process critical components can be quickly replaced during operation to minimise workflow interruptions. High grade materials and refined system design ensure precise, reliable analytical performance while simplifying maintenance. Tool free sealing structures enable fast consumable part replacement to further boost operational efficiency.
Control software supports creation, sharing and modification of experimental methods. Automated data processing delivers powerful analytical capabilities.
Product Specifications
| Operating principle & scanning wavelength | Fluorescence (280 nm excitation) plus static / dynamic light scattering (620 nm scattering); intrinsic-fluorescence spectral range 260 nm ~ 700 nm |
| Laser & low-temperature operation | Excitation laser 280 nm; scattering laser 620 nm; ice-free operation from -20 ~ 10 °C; pressure tolerance up to 5 kbar |
| Detection modes | Label-free detection; labelled detection (with dyes such as ANS, SYPRO Orange) |
| Sample temperature-control range | -20 ~ 10 °C low-temperature range; 10 ~ 95 °C high-temperature range |
| Heating rate | 0.1 ~ 10.0 °C/min; step resolution 0.05 °C |
| Number of samples per run | 1-48 samples |
| Sample volume | 2.5-50 μL |
| Measurable concentration range | 0.05 mg/mL ~ 200 mg/mL |
| Sample mass per well | 0.25 μg/well |
| Functions | Structural-stability and aggregation-stability analysis; low-/high-temperature denaturation-renaturation analysis; low-/high-temperature isothermal-stability analysis; protein colloidal-stability analysis; operation under pressure ≥ 3 kbar; particle-size analysis |
| Measurable parameters | Tagg, Tonset, Tm, ΔG, C1/2, B22/G22, kD, percentage of denatured protein, denaturation-renaturation behaviour, viscosity; particle-size intensity-weighted distribution, mass-weighted distribution, polydispersity index |
| Sample holders | Capillaries, or Corning 3540, Nunc 242764 (compatible alternatives of similar dimensions) |
| Physical dimensions | 40 cm × 43 cm × 75 cm (W × D × H) |
| Weight | 50 kg |
| Power supply | 10 A, 100-240 VAC, 50-60 Hz, single-phase |
| Software | REAL-OS control software, REAL-AS data-analysis software |
| PC requirements | Minimum two USB 2.0 ports; 3 GB RAM |
| Environmental requirements | Ambient temperature: 15 °C-40 °C; ambient humidity: 10%-75% |
Interested in ConFix?
Contact our team to learn more about how ConFix can accelerate your protein research and development.
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